Are viruses hiding within us?


Recent research results indicate that viral sequences are part of us.

Endogenous viral elements (EVEs) or endogenous retroviral elements (ERVs) have been identified in eukaryotic genomes including the human genome. The findings revealed the enormous scale of the invasion of vertebrate genomes by viral sequences. Weiss and Stoye report in a paper that was published in the Journal Science in May 2013 that retroviral genomes alone account for 6 to 14 % of the genomes analyzed to date, including approximately 8% of the human genome. Recent discoveries indicate that these endogenous retroviruses compromise more genomic DNA than that encoding the host proteome. Furthermore, the paper describes the potential consequences of the acquisition of ERVs. The following figure below illustrates this.

Functions of ERVsIt is thought that after the proviral DNA of a retrovirus integrates into the germ-line of a host it becomes inherited alongside all the host DNA sequences. The provirus may remain dormant or silent or may be expressed in the ways shown in the figure above (Source: Science 2013, vol 340 pp 820).

BSI can help you to speed up your research.

Call us at 1-800-227-0627 or visit our website at “www.biosyn.com

The consequences of the acquisition of ERVs may be neutral, detrimental, or beneficial depending on the nature of the acquired ERV and the position in which it was integrated into the genome.

These findings are insights that originated from the human genome project that were made possible by recent advancements made in instrumentation, particularly in DNA sequencing technologies. Large-scale sequencing projects have allowed comparative genomics to reveal that endogenous retroviruses (ERVs) are common parts of eukaryotic genomes. However, the discovery that approximately eight percent of the human genetic material comes from a Virus was a surprise. Mobile elements where initially discovered in 1956 by Barbara McClintock. DNA-transposons amplify without RNA intermediates, whereas retroelements rely on an RNA transcript that is retrotranscribed by a reverse transcriptase before integrating into a genome.

.

Retroviruses (RVs), such as the HIV virus, are RNA viruses that can replicate in a host cell. The replication occurs by reverse transcription in which the virus uses it own reverse transciptase, an enzyme that can make DNA from RNA, to produce DNA from its RNA genome. Reverse refers to the fact that RNA is back translated or transcript into DNA. Next, the new DNA is incorporated into the host’s genome by the enzyme integrase. The so integrated DNA is now treated by the cell as it’s on DNA or self DNA. This piece of DNA can now code for the parts needed to make the virus particles including the proteins needed for the assembly of the virus particle. The retroviruses are enveloped viruses that belong to the viral family Retroviridae. Endogenous retroviruses are special variants that are integrated into the genome of the host and are therefore inherited across generations. Note that the term “ENDOGENOUS” can refer to a substance or factor that is produced or synthesized within an organism or system such as a hormone. The data indicated that repeated integration of new viral sequences of ERVs into their host’s germline allows them to proliferate. These integrations can become fixed in the host population. The result is that ERV sequences or sequence loci now comprise 8 to 10% of the human and mouse genomes. These sequences also includes nonautonomous LTR-retrotransposons, also known as class I transposable elements. They consist of two sub-types, the long terminal repeat (LTR) and the non-LTR retrotransposons. ERVs form phylogenetically distinct lineages traditionally called “families”, each of which is thought to be the result of the expansion of a founder infection of the organism’s germline that may have occurred more than approximately 100 million years ago.

 

The majority of these proviral remnants of previous retroviral infections appear to have  been inactivated due to mutations accumulated over time, however, current research suggests that members of at least one family of human retroelements may still be capable of movement.

 

More recently scientists have found that non-retroviral viruses called bornaviruses have been endogenized repeatedly in mammals throughout evolution. Bornavirus (BDV) owes its name to the town of Borna, Germany. In the year 1885 a virus wiped out a regiment of cavalry horses. BDV can infect a range of birds and mammals, including humans. This virus is unique because it infects only neurons and can establish a persistent infection in its host’s brain. Furthermore, the entire life cycle of the virus takes place in the nucleus of the infected cells. Many endogenous Borna-like N (EBLN) elements in many diverse mammals have been identified in recent years. ERVs are considered to be a combination of pathogen and selfish genetic elements. Some ERV lineages proliferate by infecting germline cells, as do typical retroviruses, whereas others lack the env gene required for virions to enter cells and thus behave like retrotransposons.

 

Magiorkinis et al. analyzed ERV loci recovered from 38 mammal genomes by in silico screening. Modeling the relationship between proliferation and replication mechanism in detail within one group, the intracisternal A-type particles (IAPs), and performing simple correlations across all ERV lineages, the researchers could show that when ERVs lose the env gene their proliferation within that genome is boosted by a factor of 30. Next, borrowing simple epidemiological and ecological models allowed the scientists to show that retrotransposition and loss of env is the trait that leads endogenous retroviruses to becoming genomic superspreaders that take over a significant proportion of their host’s genome.

Apparently approximately half of the mammalian genome is derived from ancient transposable elements. There are two general types called (DNA)-transposons and retroelements. A more detailed classification is shown in the next figure.

Trasnposable elements classification

The majority of intragenic or near-gene insertions of retroelements appear to have been not favorable for the host and may have been either not fixed in the population or were removed from the linage in the long term. The next table shows a list of more recent insertional mutagenesis events that lead to alleles associated with diseases in humans.

.

Cases of insertional mutagenesis and recombinations caused by retroelements

.

Element

Gene

Functional role

LINE-1

Factor VIII

Hemophilia A

LINE-1

Dystrophin

Muscular dystrophy

SINE

Fukutin

Muscular dystrophy

Alu

NF1

Neurofibromatosis

LINE-1

myc*

Breast carcinoma

LINE-1

APC*

Colon cancer

LINE-1

Attractin

Soluble protein form

HERV-E

Amylase

Activation of a promoter

HERV-K

FGFR1 kinase

Myeloproliferative disorder

HERVs

AZFa region

Male infertility

FGFR1, fibroblast growth hormone receptor 1, chromosomal translocation; AZFa, azoospermia factor a, nonallelic homologous recombination between HERVs; APC, adenomatous polyposis carcinoma. *Somatic insertion.

The following table shows documented cases in which retroviral sequences have been assimilated for promoter or enhancer functions.

.

Examples of retroviral sequences involved in the regulation of cellular proteins

.

Element

Gene (promoter)

Functional role

HERV-E

Mid1

Opitz syndrome

HERV-E

Apolipoprotein C1

Liver and other tissues

HERV-E

Endothelin-B receptor

Placenta

HERV-E

Pleiotrophin

Trophoblast

HERV-L

ß1,3-galactosyltransferase

Colon, mammary gland

ERV II

BAAT (transferase)

Bile metabolism

ERV I

Aromatase

Placental estrogen synthesis

ERV III

Carbonic anhydrase 1

Erythroid carbon metabolism

LTR + LINE-2

Chaperonin

McKausick–Kaufman syndrome

HERV

INSL4 (insulin family)

Placenta

The expression of HERV sequences is suspected to cause or promote some types of cancers, however, it is also thought that they might help to regress tumors by serving as tumor-specific antigens. It has been reported that endogenous retroviral genes can code for peptides that are recognized by tumor-specific CTLs as well, at least in mice. These encoded peptides appear to be expressed in most melanomas. These peptides may serve useful for the development of therapeutic vaccines.

References

Norbert Bannert and Reinhard Kurth; Retroelements and the human genome: New perspectives on an old relation. PNAS 2004 101 (Suppl 2) 14572-14579; published ahead of print August 13, 2004, doi:10.1073/pnas.0404838101.

Cédric Feschotte. Virology: Bornavirus enters the genome. Nature, 2010; 463 (7277): 39 DOI: 10.1038/463039a

Masayuki Horie, Tomoyuki Honda, Yoshiyuki Suzuki, Yuki Kobayashi, Takuji Daito, Tatsuo Oshida, Kazuyoshi Ikuta, Patric Jern, Takashi Gojobori, John M. Coffin & Keizo Tomonaga. Endogenous non-retroviral RNA virus elements in mammalian genomes. Nature, 2010; 463 (7277): 84 DOI: 10.1038/nature08695

Gkikas Magiorkinis, Robert J. Gifford, Aris Katzourakis, Joris De Ranter, and Robert Belshaw; Env-less endogenous retroviruses are genomic superspreaders. PNAS May 8, 2012 vol. 109 no. 19 7385-7390.

Stoye JP. Endogenous retroviruses: still active after all these years? Curr Biol. 2001 Nov 13;11(22):R914-6.

Robin A. Weiss, Jonathan P. Stoye; Our Viral Inheritance. Science 17 May 2013: Vol. 340 no. 6134 pp. 820-821 DOI: 10.1126/science.1235148.  Perspective Virology.  E-mail: rweiss@ucl.ac.uk; jstoye@nimr.mrc.ac.uk.

Long Oligos BSI

BSI can help you to speed up your research.

Call us at 1-800-227-0627

or visit our website at

www.biosyn.com

.



Categories: Bioanalysis, BNA RNA, Cancer, Cancer Risk, Centromere, Chromatin, Chromosome Painting, Comparative, Comparative Genomics, Deep sequencing, DNA, DNA Analysis, DNA Editing, DNA Hybridization, DNA methylation, DNA Structure, DNA Synthesis, FISH, FISH Probes, Gene families, Genetics, Genome, Genotyping, Germline, HIV Virus, Human Genetics, Human Genome Project, Hybridization, Melanoma, Micro peptides, Molecular Probes, Next-generation sequencing, non-coding RNAs, Protein, Protein Families, Protein modifications, RNA, RNA Editing, RNA FISH, RNA Structure, RNA Synthesis, RNA World, Vaccines, Virus

Tags: , , , , , , , , , , , , , , ,

2 replies

  1. It’s going too be end of mine day, however before end I am reading this enormous paragraph to
    improve my knowledge.

  2. Excellent post. Keep writing such kind of information on your
    site. Im really impressed by it.
    Hello there, You’ve done a fantastic job. I’ll certainly digg it and for my part suggest to my friends.
    I’m sure they will be benefited from this website.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s

%d bloggers like this: