Potential Uses or Applications of the CRISPR Cas System !
Several Uses of Cas9 Induced Double Strand Breaks for Genomic Engineering as Suggested by Basset and Li in 2014 are listed below.
Most applications of CRISPR Cas9 use the induction of double strand breaks at specific sites within a genome, which can be repaired by using either non-homologous end joining (NHEJ) or homologous recombination (HR). This allows the generation of mutations and manipulation of genomes in a defined manner.
Potential uses or applications of engineered CRISPR Cas systems are:
- Regulation of transcription by interference with RNA polymerase (CRISPRi).
- Fusion to transcriptional activation (Transcriptional activation, to transcription activator VP64).
- Fusion to repression domains (Transcriptional repression, to KRAB or Krüppel associated box domain, a transcriptional repression domain).
- DNA tagging with green fluorescent protein (GFP).
- Chromatin purification using affinity tags.
- RNA recruitment by fusion to the sgRNA.
- Altering DNA topology by using dimerization domains (DNA looping).
- Chromatin modification with histone methyltransferases (HAT), demethylases (KDM) or deacetylases (HDAC) that can also be tagged to the appropriate domains.
- Knock-in/knock-out Cell Lines.
- Gene replacement.
- Gene editing including single base mutations.
- Gene tagging.
- Gene Therapy.
- Promoter modifications.
- Removal of viral sequences.
- Recombinant protein production in CHO cells.
- Disease model in human cell lines for drug discovery.
- Gene therapy in diseased cell line.
- Generation of a specific cell lines for gene function studies.
Scientists in the field of genome editing expect that this technique will change the way researchers think about and perform genetic and genomic editing and analysis.
Synthetic long RNA oligonucleotides are available at Biosynthesis Inc.
Please inquire calling 1-800-227-0627
or by clicking www.biosyn.com
Bassett, A.R., Liu, J.-L., CRISPR/Cas9 and genome editing in Drosophila, Journal of Genetics and Genomics (2014), doi: 10.1016/j.jgg.2013.12.004.
Categories: Cas9, CMR Proteins, CRISPR Cas, DNA, Epigenetics, Gene Editing, Gene Expression, Gene families, Genetics, Genome, Human Genetics, In Vitro Transcription, IVT, LincRNA, lncRNA, Long noncoding RNA, miRNA, miRNA regulation, non-coding RNAs, Nucleoprotein, Protein, Protein Families, Regulatory RNA, RNA, RNA Editing, RNA FISH, RNA Structure, RNA Synthesis, RNA World, Synthesis