Author Archives
Klaus D Linse , Ph.D
Director of Scientific Operations
klaus_linse@biosyn.com
www.biosyn.com
612 East Main Street, Lewisville, Texas 75057-4052
1-972-420-8505
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Which solid phase polypeptide synthesis do you like better? Fmoc- or tBoc-chemistry based?
It appears that Fmoc based solid phase polypeptide synthesis (SPPS) is the winner. … An evaluation of 53 different laboratories that synthesized polypeptides by members of the ‘ABRF Committee on Peptide Synthesis and Mass Spectrometry’ done in 1993 showed that… Read More ›
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The 3′ termini of mouse Piwi RNAs are 2′-O-methylated
Kirino & Mourelatos and Ohara et al. showed in 2007 that Piwi RNAs (piRNAs) are 2′-O-methylated at the 3′-end Several thousands of piwi RNA (piRNA) species that are typically 24–32 nucleotide (nt) long have been found in mammals, zebrafish, and… Read More ›
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RNA FISH can be used to visualize subcellular locations of RNA or RNA-RBP complexes including the location of noncoding RNAs !
RNA FISH allows the identification and localization of noncoding RNAs in cells ! Ribonucleic Acid Fluorescence In Situ Hybridization (RNA FISH) is a visualization technique that allows simultaneous detection, localization, and quantification of individual RNA molecules at the subcellular level…. Read More ›
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Piwi-RNAs, the Defenders of the Genome
What are Piwi- or piRNAs ? Here is the answer: P-element induced wimpy testis (Piwi)-RNAs, or Piwi-interacting RNA, or piRNAs are small RNAs typically 23 to 35 nucleotides long . . During the recent 16 years a group of small… Read More ›
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Bridged Nucleic Acid based FISH
Bridged Nucleic Acid (BNA) based FISH probes are smart tools available from Biosynthesis Inc. Recent advancements in the design and development of molecular probes and image analysis has made fluorescence in situ hybridization (FISH) a powerful tool. Although being a… Read More ›
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What is Aging? What are Aging Theories?
Have you ever asked yourself the following questions? . Why do we age? When do we start aging? Is there a biomarker for aging? Is there a limit to how old we can grow? . Many theories have been proposed… Read More ›
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DNA Methylation
What is DNA Methylation? . DNA methylation is a biochemical reaction that adds a methyl group to DNA nucleotides. The methylation of DNA has been found to alter the expression of genes in cells during development. BSI can help you… Read More ›
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Oligo Nucleotide Calculater
Oligo Designer Oligo Desinger is a comprehensive web-based tool available at Biosynthesis which calculates the various physiochemical properties of oligonucleotides based on their sequence. Listed below are the application user can access from the tool: Calculates the chemical formula Calculates… Read More ›