Author Archives

Klaus D Linse , Ph.D
Director of Scientific Operations
klaus_linse@biosyn.com
www.biosyn.com
612 East Main Street, Lewisville, Texas 75057-4052
1-972-420-8505

• Which solid phase polypeptide synthesis do you like better? Fmoc- or tBoc-chemistry based?

  By Klaus D. Linse on July 22, 2013 • ( 1 )

  

  
  

  It appears that Fmoc based solid phase polypeptide synthesis (SPPS) is the winner. … An evaluation of 53 different laboratories that synthesized polypeptides by members of the ‘ABRF Committee on Peptide Synthesis and Mass Spectrometry’ done in 1993 showed that… Read More ›

• Why should I test my CHO cell culture for virus infections?

  By Klaus D. Linse on June 27, 2013 • ( 0 )

  

  
  

  The answer is: … CHO cells can be infected in bioreactors with viruses such as Vesivirus 2117! … The presence of a virus in a bioreactor can inflict major losses for biotech and pharmaceutical companies involved in the manufacturing of… Read More ›

• The 3′ termini of mouse Piwi RNAs are 2′-O-methylated

  By Klaus D. Linse on June 14, 2013 • ( 0 )

  

  
  

  Kirino & Mourelatos and Ohara et al. showed in 2007 that Piwi RNAs (piRNAs) are 2′-O-methylated at the 3′-end Several thousands of piwi RNA (piRNA) species that are typically 24–32 nucleotide (nt) long have been found in mammals, zebrafish, and… Read More ›

• RNA FISH can be used to visualize subcellular locations of RNA or RNA-RBP complexes including the location of noncoding RNAs !

  By Klaus D. Linse on June 5, 2013 • ( 2 )

  

  
  

  RNA FISH allows the identification and localization of noncoding RNAs in cells ! Ribonucleic Acid Fluorescence In Situ Hybridization (RNA FISH) is a visualization technique that allows simultaneous detection, localization, and quantification of individual RNA molecules at the subcellular level…. Read More ›

• Piwi-RNAs, the Defenders of the Genome

  By Klaus D. Linse on June 4, 2013 • ( 0 )

  

  
  

  What are Piwi- or piRNAs ? Here is the answer: P-element induced wimpy testis (Piwi)-RNAs, or Piwi-interacting RNA, or piRNAs are small RNAs typically 23 to 35 nucleotides long . . During the recent 16 years a group of small… Read More ›

• Bridged Nucleic Acid based FISH

  By Klaus D. Linse on June 3, 2013 • ( 0 )

  

  
  

  Bridged Nucleic Acid (BNA) based FISH probes are smart tools available from Biosynthesis Inc. Recent advancements in the design and development of molecular probes and image analysis has made fluorescence in situ hybridization (FISH) a powerful tool. Although being a… Read More ›

• What are Long or large non-coding RNAs (lncRNAs)?

  By Klaus D. Linse on May 30, 2013 • ( 3 )

  

  
  

  Long or large non-coding RNAs (lncRNAs) are non-protein coding sequence transcripts ! . Long non-coding RNAs are non-protein coding sequence transcripts that contain more than 200 nucleotides. The size of these RNA molecules distinguishes them from small regulatory RNAs such… Read More ›

• What is Aging? What are Aging Theories?

  By Klaus D. Linse on May 16, 2013 • ( 1 )

  
  

  Have you ever asked yourself the following questions? . Why do we age? When do we start aging? Is there a biomarker for aging? Is there a limit to how old we can grow? . Many theories have been proposed… Read More ›

• DNA Methylation

  By Klaus D. Linse on May 15, 2013 • ( 0 )

  

  
  

  What is DNA Methylation? . DNA methylation is a biochemical reaction that adds a methyl group to DNA nucleotides. The methylation of DNA has been found to alter the expression of genes in cells during development. BSI can help you… Read More ›

• Oligo Nucleotide Calculater

  By Klaus D. Linse on May 10, 2013 • ( 0 )

  

  
  

  Oligo Designer Oligo Desinger is a comprehensive web-based tool available at Biosynthesis which calculates the various physiochemical properties of oligonucleotides based on their sequence. Listed below are the application user can access from the tool: Calculates the chemical formula Calculates… Read More ›