As of December 2013 90 solved structures were available in the PubMed structural database related to the CRISPR Cas System! -.- A few these models are shown below! A PubMed search for “CRISPR Cas” showed that close to 90 solved… Read More ›
Recently the Targeted Insertion of Short DNA Fragments into Mammalian Cells was Shown to be Possible with the help of CRISPR Cas! The direct injection of Cas9 mRNA, sgRNAs, and DNA oligos or vectors into mammalian cells was reported as… Read More ›
The CRISPR-Cas systems found in bacteria and archaea are versatile small RNAs for adaptive defense and regulation Bacteria and archaea have evolved various defense and regulatory mechanisms allowing them to react to various stressful situations caused by the environment, such… Read More ›
Circular oligonucleotides are covalently closed singular DNA or RNA strands that are resistant to degradation by many cellular DNA and RNA decay machineries. [Source: (left) Modelling DNA; Phil. Trans. R. Soc. A-2006-Harris-3319-34; (right) David Goodsell: The self-splicing RNA in PDB… Read More ›
Recent research results indicate that viral sequences are part of us. Endogenous viral elements (EVEs) or endogenous retroviral elements (ERVs) have been identified in eukaryotic genomes including the human genome. The findings revealed the enormous scale of the invasion of… Read More ›
RNA FISH can be used to visualize subcellular locations of RNA or RNA-RBP complexes including the location of noncoding RNAs !
RNA FISH allows the identification and localization of noncoding RNAs in cells ! Ribonucleic Acid Fluorescence In Situ Hybridization (RNA FISH) is a visualization technique that allows simultaneous detection, localization, and quantification of individual RNA molecules at the subcellular level…. Read More ›