Gram-negative pathogens, such as Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii are now responsible for many infections, including infections occurring in hospitals. Because of their broad spectrum of antibiotic resistance many antibiotics may now longer work well. A few years after… Read More ›
Shivarov et al. designed and validated this BNA[NC] probe bead-based assay for the Luminex Lab Scan 200 flow platform to allow for the detection and quantification of somatic mutations in Leukemia! -.- How does the assay work? Primers are designed… Read More ›
Recently the Targeted Insertion of Short DNA Fragments into Mammalian Cells was Shown to be Possible with the help of CRISPR Cas! The direct injection of Cas9 mRNA, sgRNAs, and DNA oligos or vectors into mammalian cells was reported as… Read More ›
Circular oligonucleotides are covalently closed singular DNA or RNA strands that are resistant to degradation by many cellular DNA and RNA decay machineries. [Source: (left) Modelling DNA; Phil. Trans. R. Soc. A-2006-Harris-3319-34; (right) David Goodsell: The self-splicing RNA in PDB… Read More ›
Micro RNAs (miRNAs) have recently emerged as key regulators of metabolic processes! . More and more evidence is accumulating that suggest that the dysregulation of miRNAs may contribute to metabolic abnormalities such as the misregulation of cholesterol and lipid metabolism, insulin… Read More ›
The answer is: … CHO cells can be infected in bioreactors with viruses such as Vesivirus 2117! … The presence of a virus in a bioreactor can inflict major losses for biotech and pharmaceutical companies involved in the manufacturing of… Read More ›
Long or large non-coding RNAs (lncRNAs) are non-protein coding sequence transcripts ! . Long non-coding RNAs are non-protein coding sequence transcripts that contain more than 200 nucleotides. The size of these RNA molecules distinguishes them from small regulatory RNAs such… Read More ›
