Recent research results indicate that viral sequences are part of us. Endogenous viral elements (EVEs) or endogenous retroviral elements (ERVs) have been identified in eukaryotic genomes including the human genome. The findings revealed the enormous scale of the invasion of… Read More ›
RNA
Why should I test my CHO cell culture for virus infections?
The answer is: … CHO cells can be infected in bioreactors with viruses such as Vesivirus 2117! … The presence of a virus in a bioreactor can inflict major losses for biotech and pharmaceutical companies involved in the manufacturing of… Read More ›
RNA FISH can be used to visualize subcellular locations of RNA or RNA-RBP complexes including the location of noncoding RNAs !
RNA FISH allows the identification and localization of noncoding RNAs in cells ! Ribonucleic Acid Fluorescence In Situ Hybridization (RNA FISH) is a visualization technique that allows simultaneous detection, localization, and quantification of individual RNA molecules at the subcellular level…. Read More ›
Piwi-RNAs, the Defenders of the Genome
What are Piwi- or piRNAs ? Here is the answer: P-element induced wimpy testis (Piwi)-RNAs, or Piwi-interacting RNA, or piRNAs are small RNAs typically 23 to 35 nucleotides long . . During the recent 16 years a group of small… Read More ›
Bridged Nucleic Acid based FISH
Bridged Nucleic Acid (BNA) based FISH probes are smart tools available from Biosynthesis Inc. Recent advancements in the design and development of molecular probes and image analysis has made fluorescence in situ hybridization (FISH) a powerful tool. Although being a… Read More ›
What are Long or large non-coding RNAs (lncRNAs)?
Long or large non-coding RNAs (lncRNAs) are non-protein coding sequence transcripts ! . Long non-coding RNAs are non-protein coding sequence transcripts that contain more than 200 nucleotides. The size of these RNA molecules distinguishes them from small regulatory RNAs such… Read More ›
DNA Methylation
What is DNA Methylation? . DNA methylation is a biochemical reaction that adds a methyl group to DNA nucleotides. The methylation of DNA has been found to alter the expression of genes in cells during development. BSI can help you… Read More ›
What are Micro peptides?
Micro peptides are short, unprocessed peptides with biological functions! . Do noncoding RNAs (ncRNAs) code for micro peptides? . Yes, apparently this appears to be the case! . In protein synthesis RNA acts as a template for cellular proteins and… Read More ›
What are MicroRNAs and what do they do?
MicroRNAs are small non-coding single-stranded RNA molecules MicroRNAs (miRNAs) are small non-coding single-stranded RNA molecules consisting of approximately 21 to 22 nucleotides that regulate posttranscriptional gene expression in metazoans and plants. miRNAs are usually generated from noncoding regions of gene… Read More ›
What are MicroRNA sponges?
MicroRNA sponges are decoy targets for miRNA seed families ! Ebert, Neilson & Sharp in 2007 report how to make decoy targets for several microRNA seed families, and named them ‘microRNA sponges’. Furthermore the researchers tested their ability to derepress… Read More ›