Potential Uses or Applications of the CRISPR Cas System ! -.- Several Uses of Cas9 Induced Double Strand Breaks for Genomic Engineering as Suggested by Basset and Li in 2014 are listed below. -.- Most applications of CRISPR Cas9 use… Read More ›
Synthesis
Structural Models of the CRISPR Cas System!
As of December 2013 90 solved structures were available in the PubMed structural database related to the CRISPR Cas System! -.- A few these models are shown below! A PubMed search for “CRISPR Cas” showed that close to 90 solved… Read More ›
Is Targeted Insertion of Short DNA Fragments into Mammalian Cells Possible?
Recently the Targeted Insertion of Short DNA Fragments into Mammalian Cells was Shown to be Possible with the help of CRISPR Cas! The direct injection of Cas9 mRNA, sgRNAs, and DNA oligos or vectors into mammalian cells was reported as… Read More ›
What are Circular Oligonucleotides?
Circular oligonucleotides are covalently closed singular DNA or RNA strands that are resistant to degradation by many cellular DNA and RNA decay machineries. [Source: (left) Modelling DNA; Phil. Trans. R. Soc. A-2006-Harris-3319-34; (right) David Goodsell: The self-splicing RNA in PDB… Read More ›
Peptides – a collection of words, nouns and terms to review – natural peptides, synthetic peptides, peptide mimics, artificial peptides, modified peptides and more.
WHAT ARE PEPTIDES? Peptides are macro molecules that contain amino acid monomers linked together by peptide (amide) bonds. The covalent bonds are formed when the carboxyl group of one amino acid reacts with the amino group of another. Peptides are… Read More ›
Are MicroRNAs the new players in metabolism and metabolic disorders?
Micro RNAs (miRNAs) have recently emerged as key regulators of metabolic processes! . More and more evidence is accumulating that suggest that the dysregulation of miRNAs may contribute to metabolic abnormalities such as the misregulation of cholesterol and lipid metabolism, insulin… Read More ›
What is “3-phosphoglyceryl-lysine or pgK”?
“3-phosphoglyceryl-lysine or pgK” is a newly discovered functional protein modification! The more scientists look the more they find ! . Can this new functional amino acid modification provide insights in the causes of diabetes, cancer, and other metabolic disorders in… Read More ›
Which solid phase polypeptide synthesis do you like better? Fmoc- or tBoc-chemistry based?
It appears that Fmoc based solid phase polypeptide synthesis (SPPS) is the winner. … An evaluation of 53 different laboratories that synthesized polypeptides by members of the ‘ABRF Committee on Peptide Synthesis and Mass Spectrometry’ done in 1993 showed that… Read More ›
Why should I test my CHO cell culture for virus infections?
The answer is: … CHO cells can be infected in bioreactors with viruses such as Vesivirus 2117! … The presence of a virus in a bioreactor can inflict major losses for biotech and pharmaceutical companies involved in the manufacturing of… Read More ›
What are Long or large non-coding RNAs (lncRNAs)?
Long or large non-coding RNAs (lncRNAs) are non-protein coding sequence transcripts ! . Long non-coding RNAs are non-protein coding sequence transcripts that contain more than 200 nucleotides. The size of these RNA molecules distinguishes them from small regulatory RNAs such… Read More ›
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