Long or large non-coding RNAs (lncRNAs) are non-protein coding sequence transcripts ! . Long non-coding RNAs are non-protein coding sequence transcripts that contain more than 200 nucleotides. The size of these RNA molecules distinguishes them from small regulatory RNAs such… Read More ›
Custom Long RNA
What are Micro peptides?
Micro peptides are short, unprocessed peptides with biological functions! . Do noncoding RNAs (ncRNAs) code for micro peptides? . Yes, apparently this appears to be the case! . In protein synthesis RNA acts as a template for cellular proteins and… Read More ›
What do long noncoding RNAs and Pseudogenes do?
Pseudogene long noncoding RNA networks appear to regulate transcription and translation in human cells. What are pseudogenes? Pseudogenes are now thought to be nonfunctional relatives of genes and can be defined as fragments of nucleotide sequences that resemble a domain… Read More ›
Epigenetics: Regulating Genome Integrity and Expression April 12, 2013 TWU
Biosynthesis Inc. is participating at the meeting Presented by THE FEDERATION OF NORTH TEXAS AREA UNIVERSITIES MOLECULAR BIOLOGY AND NEUROSCIENCE COMMITTEES April 12, 2013 TEXAS WOMAN’S UNIVERSITY CFO 203 Jessica Kate Tyler, Ph.D. Co-Director of the Center for Cancer Epigenetics,… Read More ›
Q-FISHing to monitor molecular aging
By Klaus D. Linse Fluorescence In Situ Hybridization (FISH) for Telomere Length Quantification or Q-FISH evolves into High-Throughput Q-FISH Fluorescence in situ hybridization (FISH) is a powerful tool and probes that exhibit high DNA and RNA affinities promise to improve… Read More ›
Get Custom Oligo Synthesis with the Best Innovative Quality
Oligonucleotide Synthesis: The Process Oligonucleotide synthesis is a simple process that has implications over vast sectors. The use of Oligonucleotide synthesis has been found useful in various laboratory procedures.Oligonucleotides are applicable in the field of medicine and molecular biology. One of the most common usages of synthetic Oligonucleotides is as an antisense Oligonucleotides, primers of DNA amplification and sequencing, for artificial gene synthesis, interfering RNA and probes from detecting complementary RNA or DNA through molecular hybridization. The Use of Creating Custom Oligonucleotide Creating a custom Oligonucleotide is useful as it binds to the region of the DNA which is complementary to the custom Oligonucleotide sequence. It is also used in making primers that are crucial in replication of PCR allowing the amplification of targeted segments in the DNA. Custom Oligonucleotide synthesis also allows restriction sites to be added to the desired Oligonucleotide. Though the length of a custom Oligonucleotide is generally 15 to 20 bases, it has the capability to limit the additional sequences that can be added to the primer sequence. The synthesis of Oligonucleotides is a chemical synthesis of fragments of nucleic acids. The synthesis is useful as it provides an inexpensive access to custom Oligonucleotides that are of the desired sequence. Chemical oligonucleotide synthesis is carried out in 3’ to 5’ direction. The enzyme synthesis, on the other hand, synthesizes RNA and DNA in a 5’ to 3’ direction. The Methodology Followed The present process uses a phosphoramidite method and is implemented as a solid phase synthesis. The building blocks are derived from protected ribonucleosides (A, C, G, and U), 2′-deoxy-nucleosides (DA, DC, dG, and T) or nucleosides which are chemically modified such as LNA. The sequential coupling of the building blocks is used to obtain the required oligonucleotide. The coupling helps the oligonucleotide chain to grow as required by the sequence of the product. When the process is completed, the product is released from the solid phase into a solution which further acts to de-protect it. The limits for the synthetic oligonucleotide length are set by the occurrence of the side reactions. This is due to the accumulation of errors during the synthesis of the oligonucleotide. The products are then isolated with the help of HPLC. This is done to ensure the obtained oligonucleotide is high in purity and is of the desired nature. The synthesized oligonucleotide is generally RNA or DNA molecule which is single stranded along with 15-25 bases in length. For more information on Long RNA, Custom Long RNA, Long RNA Synthesis, Oligonucleotides, Please Visit: Oligo Synthesis