Selected papers for CRISPR Cas! Segal and Meckler in 2013 state in ‘Genome Engineering at the Dawn of the Golden Age,’ that, “In summary, compared with ZFNs, TALENs can be designed for more targets, are more likely to be active… Read More ›
miRNA
Are MicroRNAs the new players in metabolism and metabolic disorders?
Micro RNAs (miRNAs) have recently emerged as key regulators of metabolic processes! . More and more evidence is accumulating that suggest that the dysregulation of miRNAs may contribute to metabolic abnormalities such as the misregulation of cholesterol and lipid metabolism, insulin… Read More ›
What are Nano-flares? What are the features of nano-flares?
Answer 1 . A “nano-flare” refers to a very small solar flare that happens in the corona, the external atmosphere of the Sun. . Answer 2 . Nano-flares are a new class of intracellular molecular probes. . This blog is… Read More ›
RNA FISH can be used to visualize subcellular locations of RNA or RNA-RBP complexes including the location of noncoding RNAs !
RNA FISH allows the identification and localization of noncoding RNAs in cells ! Ribonucleic Acid Fluorescence In Situ Hybridization (RNA FISH) is a visualization technique that allows simultaneous detection, localization, and quantification of individual RNA molecules at the subcellular level…. Read More ›
What are MicroRNAs and what do they do?
MicroRNAs are small non-coding single-stranded RNA molecules MicroRNAs (miRNAs) are small non-coding single-stranded RNA molecules consisting of approximately 21 to 22 nucleotides that regulate posttranscriptional gene expression in metazoans and plants. miRNAs are usually generated from noncoding regions of gene… Read More ›
What are MicroRNA sponges?
MicroRNA sponges are decoy targets for miRNA seed families ! Ebert, Neilson & Sharp in 2007 report how to make decoy targets for several microRNA seed families, and named them ‘microRNA sponges’. Furthermore the researchers tested their ability to derepress… Read More ›
A New Approach to Cancer Therapy
Poster 5319 presented by Dr. Masamitsu Konno reports a new way for a cancer therapy to treat Gastrointestinal Cancer . The title of the poster presented in the AACR 2013 Meeting poster session on Wednesday morning in the section “Non-Coding… Read More ›
What do long noncoding RNAs and Pseudogenes do?
Pseudogene long noncoding RNA networks appear to regulate transcription and translation in human cells. What are pseudogenes? Pseudogenes are now thought to be nonfunctional relatives of genes and can be defined as fragments of nucleotide sequences that resemble a domain… Read More ›
What is a Gapmer?
A gapmer is a chimeric antisense oligonucleotide that contains a central block of deoxynucleotide monomers sufficiently long to induce RNase H cleavage. . Gapmers – Design and Use The central block of a gapmer is flanked by blocks of 2’-O… Read More ›
Q-FISHing to monitor molecular aging
By Klaus D. Linse Fluorescence In Situ Hybridization (FISH) for Telomere Length Quantification or Q-FISH evolves into High-Throughput Q-FISH Fluorescence in situ hybridization (FISH) is a powerful tool and probes that exhibit high DNA and RNA affinities promise to improve… Read More ›
