Production of Antibodies

 
Producing monoclonal mouse antibodies at Abfrontier involves several key steps. Our Monoclonal antibodies are Korean laboratory-made molecules that can mimic the immune system's ability to fight off harmful pathogens such as viruses and bacteria. Here is an overview of the production process:

Antigen Selection:

Identify and select the target antigen against which the antibodies will be generated. The antigen is a specific molecule that triggers an immune response.

Immunization:

Inject mice with the chosen antigen to stimulate an immune response. The immune system of the mice will produce antibodies against the antigen.

Cell Fusion:

Isolate B cells from the immunized mice. B cells are responsible for producing antibodies. These B cells are then fused with immortalized myeloma cells to create hybridoma cells. Hybridomas have the ability to continuously produce antibodies.

Screening and Cloning:

Select hybridoma cells that produce antibodies with high specificity and affinity for the target antigen. Screening is typically done using techniques like enzyme-linked immunosorbent assay (ELISA).

Expansion of Hybridoma Cells:

Culture and expand the selected hybridoma cells in a suitable growth medium. This step is crucial to produce a sufficient quantity of monoclonal antibodies.

Antibody Purification:

Harvest the culture supernatant containing the antibodies and purify the antibodies to remove any contaminants. Common purification methods include chromatography.

Testing and Characterization:

Characterize the purified monoclonal antibodies for specificity, affinity, and functionality. Various assays are used to assess the binding properties and biological activity of the antibodies.

Scaling Up Production:

Once a viable hybridoma cell line is established and characterized, scale up the production to generate larger quantities of monoclonal antibodies.

Storage:

Store the purified antibodies without freeze thawing to maintain for stability. 

Learn more

Distributors

Discover more